Journal: PLoS Genetics
Article Title: The nanoCUT&RUN technique visualizes telomeric chromatin in Drosophila
doi: 10.1371/journal.pgen.1010351
Figure Lengend Snippet: A : schematic of the method. Relative size of the nGFPMNase (magenta) to an antibody (brown) is shown. nGFPMNase binds to the GFP (green) tag of the protein of interest (red). In the presence of Ca 2+ , MNase digests DNA (black line) that is not protected by the nucleosomes (yellow). B : landscapes of TRL isoform binding at three Hsp70 promoters. Genomic coordinates (in nt) and the scale of the hsp70 region on 3R are shown at the top. C : landscapes of preferential binding of TRL-short at the promoters of the worniu ( wor ) and snail ( sna ) genes, while an intergenic site binds both TRL isoforms (shown at the right end of the profile). Genomic coordinates and the scale of the region are shown at the top. D : consensus motifs of TRL-short sites.
Article Snippet: The plasmid for nGFPMNase expression has been deposited to Addgene (ID: 187826).
Techniques: Binding Assay